Immunoassay kits

The majority of our assays are based on a sandwich Enzyme-Linked Immunosorbent Assays (ELISA) format in which antibodies are coated on the ELISA plate, calibrators and samples containing analyte are added to the plate and the analyte is detected by an enzyme labelled anti-analyte antibody. Signal is visualised by subsequent addition of substrate and the absorbance is read on a plate reader. The intensity of signal is proportional to the concentration of the analyte in samples.

We also have an immunoturbidimetric assay, which measures turbidity of the analyte-antibody solution at 600nm and is suitable for automated analysers.

Careful consideration is given to the source of antibodies and standards used within each test; where possible we try to build our tests using species-specific antibodies and standards rarely relying on cross-reactivity between BJC_19.4.11-Avacta-0393_resizedspecies.

Each assay is fully optimised, followed by assessments of assay range, dilution linearity, matrix effect, inter- and intra-assay precision and accuracy runs. Subsequently each test is validated and has the stability assessed. Kits are also manufactured and QC tested in-house by our manufacturing laboratory. Using this rigorous approach allows us to have a tight control over our tests and means we can deliver fast and effective troubleshooting and technical advice when required.

We have a range of assays already available for veterinary market and are continuously working on new projects. All kits are sold with the minimum of 4 months expiry date. For larger orders a custom production can be arranged to guarantee longer expiry date. We also develop and manufacture SENSITEST allergy kits for partner labs and distributors worldwide. For more information please see our allergy microsite HERE.

For details on available acute phase protein kits and kit inserts see below.

Avacta canine C-Reactive Protein (Immunoturbidimetric): £750

  • Kit insert – available to download HERE
  • Liquid ready-to-use reagents
  • For use on the automated analysers
  • Assay time: <10 minutes
  • Dynamic range: 0-150mg/L
  • No sample dilution needed
  • Calibrators included in the kit
  • Three levels of matrix controls included in the kit

Avacta canine C-Reactive Protein (ELISA): £350

  • Kit insert – available to download HERE
  • Liquid ready-to-use reagents
  • 96-well plate format
  • Assay time: <1 hour
  • Dynamic range: 0.78-25ng/mL
  • Recommended sample dilution: 1 in 4,000
  • Calibrators included in the kit

Avacta canine haptoglobin (ELISA): £350

  • Kit insert – available to download HERE
  • Liquid ready-to-use reagents
  • 96-well plate format
  • Assay time: <2.5 hours
  • Dynamic range: 1.95-125ng/mL
  • Recommended sample dilution: 1 in 100,000
  • Calibrators included in the kit
  • Two levels of matrix controls included in the kit

Avacta feline Alpha-1-acid glycoprotein (AGP) (ELISA): £350

  • Kit insert – available to download HERE
  • Liquid ready-to-use reagents
  • 96-well plate format
  • Assay time: <2.5 hours
  • Dynamic range: 1.25-80ng/mL
  • Recommended sample dilution: 1 in 75,000
  • Calibrators included in the kit
  • Two levels of matrix controls included in the kit

Avacta canine Alpha-1-acid glycoprotein (AGP) (ELISA):

In development (available shortly)

Avacta feline Serum Amyloid A (SAA) (ELISA):

In development (available shortly)

Avacta canine Avacta canine Serum Amyloid A (SAA) (ELISA):

In development (available shortly)

Avacta canine Interleukin 6 (IL-6) (ELISA):

In development (available shortly)

 

To place a kit order please contact Hayley Booth on hayley.booth@avacta.com.